As a trainee, I was fortunate to have contributed to a variety of successful projects including biosynthesis of the a-factor mating pheromone (15), discovery and characterization of G-proteins (16, 17) and eukaryotic initiation factor factor eIF-4E (18) in yeast, purification and characterization of the prohormone-processing Kex2 protease (19, 20, 21,22, 23, 24), and discovery of HIT proteins as a novel superfamily of nucleotide-binding proteins (25). As an independent laboratory, we discovered that the HIT protein Hint reverses adenylyl protein modifications (26), and discovered Cdc123 as a novel cell cycle regulator (27), which interacts with Chfr-homologous FHA-RING ubiquitin ligases (28). We also characterized beta arrestin structure and function (29, 30).
We continue to look for bright and highly motivated trainees to perform interdisciplinary, high impact research.
Archival lecture notes are available for courses developed at Jefferson and Dartmouth:
- The Yeast Transformation Tutorial
- The Difference Patterson Tutorial
- The Yeast Genetics Lectures for GE 611, "Introduction to Molecular Genetics"
- The Yeast Cell Cycle and Checkpoint Lectures for GE636, "Cell Cycle, Growth Factors, Oncogenes and Tumor Suppressor Genes"
- Genetics 144, "Oncogenomics"
- Dartmouth MCB Core Course Yeast Genetics Lecture
- Those interested in obtaining the fluorescent and fluorigenic nucleotide analogs, ApppBODIPY and GpppBODIPY, described in Draganescu et al, may obtain reagents from Invitrogen.